Magnetic Proteomics Sample Prep (MPSP) Kit
Detergent/Denaturant Compatibility, Efficient On-Bead Digestion, Comprehensive Proteome Coverage
- Use any lysis buffer, including detergents or denaturants, for seamless workflow compatibility
- Maximize recovery of scarce proteins that are crucial for comprehensive proteome analyses
- Streamline your workflows with automation-friendly design, surpassing traditional methods like precipitation and filtration
- Leverage superior magnetic response to competing beads for effective and efficient bead handling
Catalog Number:
Size
Catalog Number: CS3325A04
Simplify Protein Sample Preparation with Magnetic Beads for Bottom-Up Proteomics
Magnetic Proteomics Sample Prep (MPSP) Beads offer a solution for efficient, reliable protein sample preparation in bottom-up proteomics workflows. Designed for consistent and comprehensive proteome coverage, MPSP beads excel in compatibility with various buffers, including those containing detergents or denaturants, ensuring seamless integration into diverse workflows. Their superior magnetic response enables efficient bead handling, outperforming competing technologies and enhancing usability.
Optimized for the recovery of low-abundance proteins, MPSP magnetic beads help achieve deeper proteome insights critical for comprehensive analyses. These beads enable efficient on-bead digestion, minimizing sample loss and maximizing reproducibility. Automation-friendly and scalable, they offer a streamlined alternative to traditional methods like precipitation, FASP and S-Trap, making them ideal for both manual and automated platforms.
By incorporating MPSP beads into proteomics workflows, you can achieve high-quality, reproducible data with enhanced flexibility and efficiency, empowering discoveries across a wide range of applications.
Recovery of digested peptides after capture using Promega MPSP beads compared to recovery after capture using beads from another key supplier. Protein extracted from Human K562 cells using the Lysis Buffer (Whole Cell) was combined with Promega MPSP Magnetic Beads (1:10, Protein: Bead ratio) and acetonitrile (80% final concentration). The beads were incubated for 20 minutes with shaking at 1200rpm, magnetically separated, washed 3X with 80% ethanol and digested overnight using the Digestion Buffer containing Trypsin/Lys-C Mix (1:20 Enzyme: Substrate ratio). Peptide concentrations were measured using the Thermo Scientific Pierce quantitative colorimetric peptide assay using the Promega K562 peptide digest as the peptide standard (spiked into the prototype digestion buffer).
Identified Proteins
Total Peptides
Digestion Efficiency
Comparative proteomic analysis of K562 proteins using different magnetic beads. Digested peptides were loaded onto a 0.075 × 50cm C18 column (Acclaim PepMAP (Thermo)) with a 2–32% B (80% acetonitrile) gradient over 2 hours using a vanquish neo LC system (Thermo). Mass spectral data were collected using a cycle time of 1 second in a data-dependent mode with a Thermo exploris 240 mass spectrometer (Thermo). Raw files were searched with proteome discover 3.1 using Sequest as a search engine (precursor detector included) against the Human Uniprot database. All data were filtered using a 1% protein and 0.1% peptide FDR.
Bead Comparison
Desalting Comparison
Bead capture is surface-independent and proteins overlap across desalting methods. Panel A. Venn analysis comparing three bead types. The central section represents proteins common to all bead types, indicating that greater than 95% of the proteins identified are common to all 3 bead types and suggesting that bead capture is not predisposed to any surface type. Panel B. Venn analysis comparing desalting using three common approaches, MPSP bead capture, strap and precipitation. This analysis suggests that the majority of proteins (about 83%) are found using all three methods. A small subset of proteins (about 7%) are unique to each workflow. Both analyses consisted of six replicates.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lysis Buffer (Whole Cell) |
CS3325A01 | 1 × 25ml | |
Digestion Buffer |
CS3325A05 | 1 × 25ml | |
MPSP Magnetic Beads Slurry |
CS3325A03 | 1 × 5ml | 20mg/ml in 20% ethanol |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to
- (1) use in further material manufacture; and
- (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
Related Products
Similar Products
Magne® Protein G and Magne® Protein A Beads
Magnetic affinity beads with Protein A or G for Ab purification from culture media, ascites and serum samples.
G7471, G7472, G7473, G8781, G8782, G8783
High Capacity Magne® Streptavidin Beads
Magnetic affinity beads for binding biotinylated antibodies and proteins from complex biological samples with low nonspecific binding.
V7820, V7830
Frequently Used With
Trypsin Platinum, Mass Spectrometry Grade
Maximum digest specificity. Maximum resistance to autoproteolysis.
VA9000
Rapid-Digestion Trypsin and Trypsin/Lys-C Kits
Streamlined protocol for fast, efficient protein digestion.
VA1060, VA1061
Sequencing Grade Modified Trypsin
Highly active and stable trypsin manufactured to provide maximum specificity.
V5111, V5117, V5113
Trypsin Gold, Mass Spectrometry Grade
Maximum digest specificity with extreme resistance to autolytic digestion.
V5280
Trypsin/Lys-C Mix, Mass Spec Grade
Increased peptide recovery, enhanced protein quantitation, improved reproducibility.
V5071, V5072, V5073
Arg-C Ultra, Mass Spec Grade
Endopeptidase that cleaves at the C-terminus of arginine residues, including Arg-Pro.
VA1831, VA1832
ProAlanase (Mass Spec Grade)
An endoprotease that preferentially cleaves proteins on the C-terminal side of proline and alanine.
VA2161, VA2171
Lys-C, Mass Spec Grade
Cleavage site: C-terminal of Lys. Optimal pH: 7–9.
VA1170
rAsp-N, Mass Spec Grade
Cleavage site: N-terminal of Asp. Optimal pH: 6–9.
VA1160
